KMID : 0385520110240030231
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Analytical Science & Technology 2011 Volume.24 No. 3 p.231 ~ p.235
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Spectrofluorimetric determination of EDTA with Cu(¥±)-tiron chelate
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Choi Hee-Seon
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Abstract
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A spectrofluorimetric method for the determination of EDTA in real samples such as mayonnaise, powder detergent and cleansing cream with tiron (4,5-dihydroxy-1,3-benzenedisulfonic acid) as a fluorimetric reporter was developed. When tiron is chelated with Cu(¥±), the fluorescent intensity is decreased by a quenching effect. However, when Cu(¥±)-tiron chelate reacts with EDTA, fluorescent intensity is increased as tiron is released. Several experimental conditions such as pH of the sample solution, the amount of Cu(¥±), the amount of tiron, heating temperature and heating time were optimized. Fe(¥²) interfered more seriously than any other ions, interference of Fe(¥²) could be disregarded, because Fe(¥²) was scarcely contained in selected real samples. The linear range of EDTA was from 8.0¡¿10?? M to 2.0¡¿10?? M. With this proposed method, the detection limit of Fe(¥²) was 5.2¡¿10?? M. Recovery yields of 92.7~99.3% were obtained. Based on experimental results, it is proposed that this technique can be applied to the practical determination of EDTA.
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KEYWORD
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EDTA determination, tiron, Cu(¥±), spectrofluorimetry
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